Coding
RFP-BA-Bsa

Part:BBa_K349002:Design

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)

RFP-BA-BsaI (for construction of BA BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1086


Design Notes

Cutting at the BsaI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioByte assembly method of Team Alberta 2010.

Source

pSB1C3

References